Microbiological Testing

I. Bactericidal activity

The Company "Capital diagnostics" in Scotland, UK has performed tests on two isolated bacteria from the uterus of mares. Here are the results.

S. zooepidemicus

Number of bacteria

	Exposure time
Dilution	30 s	1 min	5 min	10 min	20 min
10²	0	0	0	0	0
10³	0	0	0	0	0
10⁴	0	0	0	0	0
10⁵	0	0	0	0	0

Viable numbers of bacteria to the test> 5000 cfu / ml beginning

E. coli

Number of bacteria

	Exposure time
Dilution	30 s	1 min	5 min	10 min	20 min
10²	0	0	0	0	0
10³	0	0	0	0	0
10⁴	0	0	0	0	0
10⁵	0	0	0	0	0

Viable numbers of bacteria to the test> 5000 cfu / ml beginning

II. Bactericidal activity

The laboratory and the Medical Institute of the University of Nagasaki, Japan microbiological tests of the effectiveness of the anode liquid against the most common pathogens Staphylococcus aureus, Staphylococcus epermidis, Serratia marcencens, Escherichia coli, Pseudomonas auruginosa and Burkholderia cepacia under a concentration of about 1.000. 000 cfu / ml carried out. Anolyte was estimated in terms of three specific conventional means: 0.1% chlorhexidine (Herbitane solution, Ici-pharma, Osaka, Japan), 0.02% to povidine iodine (Isodine solution, Meiji Seika, Tokyo) and 80% ethanol (ethanol pour désinfection, Maruisha Pharmaseutical Co . Ltd., Osaka).These drugs are often used at the same concentrations in the products for disinfection of the hands. All disinfectant solutions were mixed with sterile distilled water. Sterile distilled water was used as to the precise execution of the test. The results are shown in the following table.

Bacteria	Disinfectant	10 s	60 s	180 s
MSSA	Anolyte	0	0	0
methicillin	0.02% Povidine iodine	0	0	0
sensitive	80% Ethanol	0	0	0
Staphylococcus	0.1% chlorhexidine	>500	>500	>500
aureus	Control: distilled water	>500	>500	>500
MRSA	Anolyte	0	0	0
methicillin	0.02% Povidine iodine	0	0	0
resistant	80% Ethanol	0	0	0
Staphylococcus	0.1% chlorhexidine	>500	>500	>500
aureus	Control: distilled water	>500	>500	>500
Staphylococcus	Anolyte	0	0	0
epedermis	0.02% Povidine iodine	0	0	0
	80% Ethanol	0	0	0
	0.1% chlorhexidine	>500	>500	0
	Control: distilled water	>500	>500	>500
Pseudomonas	Anolyte	0	0	0
aeruginosa	0.02% Povidine iodine	0	0	0
	80% Ethanol	0	0	0
	0.1% chlorhexidine	41	0	0
	Control: distilled water	>500	>500	>500
Escheria coli	Anolyte	0	0	0
	0.02% Povidine iodine	0	0	0
	80% Ethanol	0	0	0
	0.1% chlorhexidine	0	0	0
	Control: distilled water	>500	>500	>500
Serratia	Anolyte	0	0	0
marcencens	0.02% Povidine iodine	0	0	0
	80% Ethanol	0	0	0
	0.1% chlorhexidine	>500	27	0
	Control: distilled water	>500	>500	>500
Burkholderia	Anolyte	0	0	0
cepacia	0.02% Povidine iodine	0	0	0
	80% Ethanol	0	0	0
	0.1% chlorhexidine	>500	>500	>500
	Control: distilled water	>500	>500	>500

It is noted that the performance of anolyte is comparable to that of ethanol, which was diluted to 80% in distilled water. Anolyte is however better than the two other disinfectant of this test. One can therefore conclude that anolyte is a particularly powerful, inexpensive disinfectant.

III. Sporicide activity

The City Hospital NHS Trust, Brimmingham, UK, has carried out tests on sporicide activity of anolyte. The following table compares anolyte with glutaraldehyde.

log₁₀ spores that exist for anolyte or 2% glutaraldehyde remain

Contact time	Anolyte	2% glutaraldehyde
Pre disinfection challenge	7.76	7.76
1 min	4.84	7.63
2 min	2.34	7.60
5 min	1.30	7.46
10 min	0	7.19
20 min	0	6.87
30 min	0	6.34
1 hour	0	2.75
2 hours	0	0

Anolyte is therefore also suitable for sporicide. A reduction of 6 is log₁₀ in 5 minutes before, which is by far better than the 2% of glutaraldehyde.

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