Envirolyte Egypt

Microbiological Testing

I. Bactericidal activity

The Company "Capital diagnostics" in Scotland, UK has performed tests on two isolated bacteria from the uterus of mares. Here are the results.

S. zooepidemicus

Number of bacteria

Exposure time
Dilution 30 s 1 min 5 min 10 min 20 min
102 0 0 0 0 0
103 0 0 0 0 0
104 0 0 0 0 0
105 0 0 0 0 0

Viable numbers of bacteria to the test> 5000 cfu / ml beginning

E. coli

Number of bacteria

Exposure time
Dilution 30 s 1 min 5 min 10 min 20 min
102 0 0 0 0 0
103 0 0 0 0 0
104 0 0 0 0 0
105 0 0 0 0 0

Viable numbers of bacteria to the test> 5000 cfu / ml beginning

II. Bactericidal activity

The laboratory and the Medical Institute of the University of Nagasaki, Japan microbiological tests of the effectiveness of the anode liquid against the most common pathogens Staphylococcus aureus, Staphylococcus epermidis, Serratia marcencens, Escherichia coli, Pseudomonas auruginosa and Burkholderia cepacia under a concentration of about 1.000. 000 cfu / ml carried out. Anolyte was estimated in terms of three specific conventional means: 0.1% chlorhexidine (Herbitane solution, Ici-pharma, Osaka, Japan), 0.02% to povidine iodine (Isodine solution, Meiji Seika, Tokyo) and 80% ethanol (ethanol pour désinfection, Maruisha Pharmaseutical Co . Ltd., Osaka).These drugs are often used at the same concentrations in the products for disinfection of the hands. All disinfectant solutions were mixed with sterile distilled water. Sterile distilled water was used as to the precise execution of the test. The results are shown in the following table.

Bacteria Disinfectant 10 s 60 s 180 s
MSSA Anolyte 0 0 0
methicillin 0.02% Povidine iodine 0 0 0
sensitive 80% Ethanol 0 0 0
Staphylococcus 0.1% chlorhexidine >500 >500 >500
aureus Control: distilled water >500 >500 >500
MRSA Anolyte 0 0 0
methicillin 0.02% Povidine iodine 0 0 0
resistant 80% Ethanol 0 0 0
Staphylococcus 0.1% chlorhexidine >500 >500 >500
aureus Control: distilled water >500 >500 >500
Staphylococcus Anolyte 0 0 0
epedermis 0.02% Povidine iodine 0 0 0
80% Ethanol 0 0 0
0.1% chlorhexidine >500 >500 0
Control: distilled water >500 >500 >500
Pseudomonas Anolyte 0 0 0
aeruginosa 0.02% Povidine iodine 0 0 0
80% Ethanol 0 0 0
0.1% chlorhexidine 41 0 0
Control: distilled water >500 >500 >500
Escheria coli Anolyte 0 0 0
0.02% Povidine iodine 0 0 0
80% Ethanol 0 0 0
0.1% chlorhexidine 0 0 0
Control: distilled water >500 >500 >500
Serratia Anolyte 0 0 0
marcencens 0.02% Povidine iodine 0 0 0
80% Ethanol 0 0 0
0.1% chlorhexidine >500 27 0
Control: distilled water >500 >500 >500
Burkholderia Anolyte 0 0 0
cepacia 0.02% Povidine iodine 0 0 0
80% Ethanol 0 0 0
0.1% chlorhexidine >500 >500 >500
Control: distilled water >500 >500 >500

It is noted that the performance of anolyte is comparable to that of ethanol, which was diluted to 80% in distilled water. Anolyte is however better than the two other disinfectant of this test. One can therefore conclude that anolyte is a particularly powerful, inexpensive disinfectant.

III. Sporicide activity

The City Hospital NHS Trust, Brimmingham, UK, has carried out tests on sporicide activity of anolyte. The following table compares anolyte with glutaraldehyde.

log10 spores that exist for anolyte or 2% glutaraldehyde remain

Contact time Anolyte 2% glutaraldehyde
Pre disinfection challenge 7.76 7.76
1 min 4.84 7.63
2 min 2.34 7.60
5 min 1.30 7.46
10 min 0 7.19
20 min 0 6.87
30 min 0 6.34
1 hour 0 2.75
2 hours 0 0

Anolyte is therefore also suitable for sporicide. A reduction of 6 is log10 in 5 minutes before, which is by far better than the 2% of glutaraldehyde.

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